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KMID : 1101320080400010001
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Korean Journal of Clinical Laboratory Science
2008 Volume.40 No. 1 p.1 ~ p.5
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Identification of Nontuberculous Mycobacteria Existing in Public Bathroom Water by PCR-Restriction Fragment Length Polymorphism
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Choi Seung-Gu
Kang Chee-Hwan
Cho Kyu-Bong
Lee Jae-Sang
Lee Jang-Ho
Kim Sung-Il
Jee Soo-Il
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Abstract
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Thirty two of bathroom water samples from public bathroom in Seoul areas were examined using acid-fast staining, L?wenstein-Jensen (L-J) medium culture and PCR-restriction fragment length polymorphism (PCR-RFLP). In 6.25% (2/32) bathroom water samples, acid-fast bacilli were detected by AFB stain, and in 21.9% (7/32) bathroom water samples, acid fast bacilli grew on L-J media. Of them, six acid-fast bacilli were identified as Mycobacterium avium, and the other AFB as Mycobacterium szulgai by PCR-RFLP. These results are suggested that accidental nontuberculosis mycobacterial infection to a weakness person will be possible in public area.
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KEYWORD
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Public bathroom water, Nontuberculous mycobacteria, PCR-RFLP
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